Objectives

Describe the theory behind the various sequencing technologies
Differentiate between the major sequencing technologies
Determine the proper sequencing technology for a given project
Understand basic phylogenetic trees (more in the next lesson)
Analyze molecular epidemiologic data

To Do List:

Take the pre-lesson assessment
Watch summary lecture video
Watch NGS overview video video
Watch Sanger sequencing video
Watch Pyrosequencing video
Watch IonTorrent video
Watch Illumina video
Watch PacBio video
Watch NanoPore video
Complete post-lesson assessment

Complete Pre-Lesson Assessment

Start Assessment

Signal Amplification Lecture by Cullen Lilley

NGS Overview by Dr. Chow from iBiology

https://www.ibiology.org/techniques/next-generation-sequencing Next generation sequencing allows DNA samples to be sequenced quickly and affordably. Learn ho...

Sanger Sequencing

This is an animated video on DNA sequencing by Sanger's method. Sanger sequencing method is based the use of dideoxy nucleotides in the DNA polymerizing reac...

Pyrosequencing Video

Animation on QIAGEN's Pyrosequencing system and the 5-step reaction cascade.

IonTorrent Video

Illumina Overview

Explore the Illumina workflow, including sequencing by synthesis (SBS) technology, in 3-dimensional detail. Go from sample preparation, to cluster generation...

PacBio Sequencing

PacBio sequencing instruments are powered by Single Molecule, Real-Time (SMRT) Sequencing technology. Learn how to go from DNA to discovery and explore the v...

NanoPore Sequencing

image in just few decades to come a person reading his or her DNA code like as if you're reading a text sms on your mobile phone, that tell you what to do or...

Molecular Epidemiology Lecture

Listen to this Episode of Meet the Microbiologist

(Required Info for Exam)

Congratulations on finishing this module! In order to get your course certificate, please complete the exam and satisfaction survey linked below.

The exam will consist of 10 questions with a short feedback section at the beginning. You will have TWO attempts to take the quiz and pass with a score of 60% or higher. If you do not receive your certificate with 10 minutes of completing your exam, please contact us.